CD47 Protein (C-human IgG1-Fc, Human)

1.Immobilized mouse SIRPA-His (Cat:50956-M08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.05-0.13 μg/ml (Routinely tested) . 2.Immobilized human SIRPG-His (Cat:11828-H08H) at 10 μg/ml (100 μl/well) can bind human CD47-Fc, The EC50 of human CD47-Fc is 0.58-1.34 μg/ml (Routinely tested) . 3.Immobilized Recombinant Human CD47 Protein (ECD, Fc Tag) (Cat: 12283-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human SIRP alpha / CD172a Protein (ECD, His Tag) (Cat: 11612-H08H), the EC50 is 20-60 ng/mL (QC tested) . 4.Loaded Recombinant Human CD47 Protein, hFc Tag (Cat. No. 12283-H02H) on proA Biosensor, can bind Recombinant Human SIRP alpha/SHPS-1 Protein, His Tag (Cat. No. 11612-H08H) with an affinity constant of 0.693 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human CD47/Fc is a disulfide-linked homodimer. The reduced monomer comprises 362 amino acids and has a predicted molecular mass of 40.7 kDa. The apparent molecular mass of the protein is approximately 48-60 kDa in SDS-PAGE under reducing conditions due to glycosylation.