CD47 Protein (C-His, Mouse)

1.Measured by its ability to bind mouse SIRPA-Fc (Cat:50956-M02H) in functional ELISA. 2.Measured by its binding ability in a functional ELISA. 3.Immobilized mouse CD47-His (Cat:57231-M08H) at 10μg/mL (100μL/well) can bind mouse SIRPA-Fc (Cat:50956-M02H), the EC50 of mouse SIRPA-Fc is 0.07-0.3μg/mL. 4.Recombinant Mouse SIRP alpha/SHPS-1 Protein, hFc Tag (Cat. No. 50956-M02H) captured on Protein A chip can bind Recombinant Mouse CD47 Protein, His Tag (Cat. No. 57231-M08H) with an affinity constant of 0.5703 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant mouse CD47 consists of 133 amino acids and predicts a molecular mass of 15.3 kDa. As a result of glycosylation, the apparent molecular mass of mouse SIRPA is approximately 34-44 kDa in SDS-PAGE under reducing conditions.