CD47 Protein (C-His, Human)
Product Specifications
Abbreviation
CD47
Synonyms
IAP, MER6, OA3
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Human SIRP alpha hFc (Cat: 11612-H02H1) at 2 μg/ml (100 μl/well) can bind Human CD47 His (Cat: 12283-H08H1), the EC50 is 60-210 ng/mL (QC tested) . 2.Loaded Anti-Human CD47 antibody, human IgG4 on ProA Biosensor, can bind Recombinant Human CD47 Protein, His Tag (Cat. No. 12283-H08H1) with an affinity constant of 4.6nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Anti-CD47 (Research Grade Magroli*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein (His Tag) (Cat: 12283-H08H1), the EC50 is 1.6 ng/mL (Routinely tested) .
Function
Gln19-Pro139
Molecular Weight
The recombinant human CD47 consists of 132 amino acids and predicts a molecular mass of 15.17kDa. It migrates as an approximately 34.13 kDa band in SDS-PAGE under reducing conditions.
Species
Human
Curated Selection
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