CD47 Protein (C-His, Human)

1.Immobilized Human SIRP alpha hFc (Cat:11612-H02H1) at 2 μg/ml (100 μl/well) can bind Human CD47 His (Cat:12283-H08H), the EC50 of Human CD47 His is 5.0-25.0 ng/mL (QC tested) . 2.Captured Anti-CD47 (Research grade Ligufalimab biosimilar) (IgG4) on proA Chip can bind CD47 (Cat.No.12283-H08H) with an affinity constant of 10.82 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 3.Loaded Anti-CD47 (Research grade Hu5F9-G4 biosimilar) (IgG4) on ProA Biosensor, can bind human CD47 protein, His Tag (Cat.No.12283-H08H) with an affinity constant of 3.02nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 4.Immobilized Anti-CD47 (Research Grade Magroli*mab Biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD47 Protein (His Tag) (Cat: 12283-H08H1), the EC50 is 1.6 ng/mL (Routinely tested) . 5.Immobilized Recombinant Human CD47 Protein (ECD, His Tag) (Cat: 12283-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human SIRP gamma Protein (Fc Tag) (Cat: 11828-H02H), the EC50 is 100-300 ng/mL (Routinely tested) .

The recombinant human CD47 consists of 132 amino acids and predicts a molecular mass of 15.2 kDa. The apparent molecular mass of the protein is approximately 35-44 kDa in SDS-PAGE under reducing conditions due to glycosylation.