CD40LG Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CD40LG

Synonyms

CD154, CD40-L, CD40L, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1. Immobilized human CD40 at 2 μg/ml (100 μl/well) can bind human CD40L Fc Chimera with a linear range of 1.28-32 ng/ml. 2. Measured by its ability to induce CD40 pathway activation in a Luciferase receptor Assay System. The IC50 for this effect is 10-100 ng/mL. 3.Loaded Recombinant Human CD40 Protein, His Tag (Cat.No.10774-H08H) on His1K Biosensor, can bind Recombinant Human CD40 Ligand Protein, hFc Tag (Cat.No.10239-H01H) with an affinity constant of 5.88nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested) .

Function

Met113-Leu261

Molecular Weight

The recombinant human CD40L/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 386 amino acids and has a predicted molecular mass of 42.8 kDa. The apparent molecular mass of rh CD40L/Fc monomer is approximately 47.8 kDa in SDS-PAGE under reducing conditions due to glycosylation.

Species

Human