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CD40 Protein (C-mouse IgG2a-Fc, Human)

Product Specifications

Abbreviation

CD40

Synonyms

Bp50, CDW40, p50, TNFRSF5

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 0.05 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human CD40 / TNFRSF5 Protein (mFc Tag) (Cat: 10774-H38H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD40L/CD154/TNFSF5 Protein (Fc&AVI Tag), Biotinylated (Cat: 10239-H42H-B), the EC50 is 8-25 ng/mL (Routinely tested) . 2.Loaded Recombinant Human CD40 / TNFRSF5 Protein (mFc Tag) (Cat. No. 10774-H38H-UE) on ProA Biosensor, can bind Recombinant Human CD40L / CD154 / TNFSF5 Protein (His Tag) (Cat. No. 10239-H08E) with an affinity constant of 0.33 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Arg193

Molecular Weight

The recombinant human CD40 consists of 406 amino acids and predicts a molecular mass of 45.5 kDa. The molecular weight of this protein is around 103.5 kDa verified by SEC-MALS (Routinely tested) .

Species

Human

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