CD40 Protein (C-mouse IgG2a-Fc, Human)

Product Specifications

Abbreviation

CD40

Synonyms

Bp50, CDW40, p50, TNFRSF5

Host

HEK 293

Tag

C-mouse IgG2a-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Human CD40 / TNFRSF5 Protein (Fc Tag) (Cat: 10774-H38H) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD40L/CD154/TNFSF5 Protein (Fc&AVI Tag), Biotinylated (Cat: 10239-H42H-B), the EC50 is 7-21 ng/mL (Routinely tested) . 2.Loaded Recombinant Human CD40/TNFRSF5 Protein, mFc Tag (Cat. No. 10774-H38H) on proA Biosensor, can bind Recombinant Human CD40 Ligand/TNFSF5 Protein, His Tag (Cat. No. 10239-H08E) with an affinity constant of 7.35 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human CD40L / CD154 / TNFSF5 Protein (His Tag) (Cat: 10239-H08E) at 5 μg/mL (100 μL/well) can bind Recombinant Human CD40 / TNFRSF5 Protein (Fc Tag) (Cat: 10774-H38H), the EC50 is 200-600 ng/mL (QC tested) .