CD40 Protein (C-His, Human)

1.Immobilized Human CD40 His (Cat: 10774-H08H) at 2 μg/mL (100 μL/well) can bind Human CD40 Ligand (ECD, hFc & AVI Tag), Biotinylated (Cat: 10239-H42H-B), the EC50 is 6-20 ng/mL (QC tested) . 2.Loaded Recombinant Human CD40 Protein, His Tag (Cat.No.10774-H08H) on His1K Biosensor, can bind Recombinant Human CD40 Ligand Protein, hFc Tag (Cat.No.10239-H01H) with an affinity constant of 5.88nM as determined in BLI assay (Sartorius Octet RH16) (Routinely tested) . 3.Immobilized Recombinant Human CD40 / TNFRSF5 Protein (His Tag) (Cat: 10774-H08H) at 1 μg/mL (100 μL/well) can bind Anti-CD40 (Research Grade FFP-104 Biosimilar), the EC50 is 4.5 ng/mL (Routinely tested) .

The secreted recombinant human CD40 comprises 184 amino acids with a predicted molecular mass of 20.7 kDa. As a result of glycosylation, rhCD40 migrates as an approximately 32 kDa band in SDS-PAGE under reducing conditions.