CD4 Protein (C-His, Human)

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When 5x10^4 cells/well are added to CD4-coated plates (0.8 μg/mL and 100 μl/well), approximately >30 % will adhere specifically after 30 minutes at 37°C. 2.Immobilized Ibalizumab-Derived Anti-CD4 Antibody, Human IgG4 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD4 / LEU3 Protein (aa 1-208, His Tag) (Cat: 10400-H08H1), the EC50 is 0.5-2.5 ng/mL (QC tested) .

The secreted recombinant human CD4 (aa 1-208) consists of 194 amino acids and has a predicted molecular mass of 21.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD4 is approximately 26 kDa.