CD3D & CD3E Protein (C-His, Human)

Product Specifications

Abbreviation

CD3D & CD3E

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (His Tag) (Cat: CT038-H08H1) at 1 μg/mL (100 μL/well) can bind OKT3-Derived Anti-CD3 antibody, Mouse IgG2a, the EC50 is 2-8 ng/mL (QC tested) . 2.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (His Tag) (Cat: CT038-H08H1) at 1 μg/mL (100 μL/well) can bind SP34-Derived Anti-CD3 antibody, Human IgG1, the EC50 is 4-12 ng/mL (Routinely tested) . 3.Recombinant Human CD3D & CD3E Heterodimer Protein, His Tag (Cat. No. CT038-H08H1) captured on CM5 Chip via Anti-his antibody chip, can bind mOKT3 with an affinity constant of 6.604 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 4.SP34-derived anti-CD3E antibody, human IgG1 captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human CD3D & CD3E Heterodimer Protein (His Tag) (Cat: CT038-H08H1) with an affinity constant of 3.820 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Function

Met1-Ala105 & Met1-Asp126

Molecular Weight

The recombinant Human CD3D & CD3E Heterodimer has a calculated molecular mass of 29.99 (13.35+16.64) KDa. The apparent molecular mass of the recombinant protein is approximately 27.3、23.7 and 21.3 KDa respectively in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 36.5 kDa verified by SEC-MALS (Routinely tested) .

Species

Human