CD276 Protein (C-His, Cynomolgus)

Product Specifications

Abbreviation

CD276

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Anti-B7-H3 (Research Grade Enoblituzumab biosimilar) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus B7-H3 / CD276 Protein (ECD, His Tag) (Cat: 90806-C08H), the EC50 is 2.7-9 ng/mL (QC tested) . 2.Immobilized Recombinant Cynomolgus B7-H3 / CD276 Protein (ECD, His Tag) (Cat: 90806-C08H) at 2 μg/mL (100 μL/well) can bind anti-B7-H3 (Research Grade Enoblituzumab biosimilar), the EC50 is 2.6 ng/mL (Routinely tested) .

Function

Met1-Glu465

Molecular Weight

The recombinant cynomolgus CD276 consists of 448 amino acids and predicts a molecular mass of 48.5 kDa. As a result of glycosylation, it migrates as an approximately 68.3 kDa band in SDS-PAGE under reducing conditions.