CD274 Protein (C-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

CD274

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

1.Measured by its ability to bind Cynomolgus PD1-Fc (Cat: 90311-C02H) in a functional ELISA. 2.Immobilized Anti-PD-L1 Antibody, Human IgG1 (Atezolizumab) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus PD-L1 / B7-H1 / CD274 Protein (His Tag) (Cat: 90251-C08H), the EC50 is 0.6-2 ng/mL (QC tested) .

Function

Met1-Thr239

Molecular Weight

The recombinant cynomolgus / rhesus CD274 comprises 232 amino acids and has a calculated molecular mass of 26.7 KDa. As a result of glycosylation, it migrates as an approximately 37.7 kDa band in SDS-PAGE under reducing conditions.