CD274 Protein (C-His, Mouse)

1.Loaded Recombinant Mouse PD-L1/B7-H1 Protein , His Tag (Cat. No. 50010-M08H) on HIS1K Biosensor, can bind Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) with an affinity constant of 0.519μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Recombinant Mouse PD-1 Protein , hFc Tag (Cat. No. 50124-M02H) captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag (Cat. No. 50010-M08H) with an affinity constant of 2.489 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Mouse PD-L1/B7-H1 Protein, His Tag (Cat. No. 50010-M08H) captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) with an affinity constant of 1.117 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 43-55 kDa band in SDS-PAGE under reducing conditions.