What is the catalog number for CD274 Protein (C-His, Mouse)?

The catalog number for CD274 Protein (C-His, Mouse) is P520776.

What sizes are available for CD274 Protein (C-His, Mouse)?

CD274 Protein (C-His, Mouse) is available in the following size(s): 100 µg.

Is CD274 Protein (C-His, Mouse) in stock?

CD274 Protein (C-His, Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

CD274 Protein (C-His, Mouse)

Product Specifications

Abbreviation

CD274

Synonyms

A530045L16Rik, B7h1, Cd274, Pdcd1l1, Pdcd1lg1, Pdl1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 98 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

1.Loaded Recombinant Mouse PD-L1/B7-H1 Protein , His Tag (Cat. No. 50010-M08H) on HIS1K Biosensor, can bind Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) with an affinity constant of 0.519μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Recombinant Mouse PD-1 Protein , hFc Tag (Cat. No. 50124-M02H) captured on Protein A chip can bind Recombinant Mouse PD-L1/B7-H1, His Tag (Cat. No. 50010-M08H) with an affinity constant of 2.489 μM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Mouse PD-L1/B7-H1 Protein, His Tag (Cat. No. 50010-M08H) captured on CM5 chip via Anti-his antibody, can bind Recombinant Mouse PD-1 Protein, hFc Tag (Cat. No. 50124-M02H) with an affinity constant of 1.117 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

Function

Met1-Thr238

Molecular Weight

The secreted recombinant mouse CD274 comprises 231 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, it migrates as an approximately 43-55 kDa band in SDS-PAGE under reducing conditions.