CD274 Protein (C-His, Canine)

1.Immobilized Recombinant Canine PD-L1/B7-H1 Protein (ECD, His Tag) (Cat: 70110-D08H) at 2 μg/mL (100 μL/well) can bind Recombinant Canine PD1 / PDCD1 / CD279 Protein (Fc Tag) (Cat: 70109-D02H), the EC50 is 500-1400 ng/mL (Routinely tested) . 2.Loaded Recombinant Canine PD-L1/B7-H1 Protein, His Tag (Cat. No. 70110-D08H) on NTA Biosensor, can bind Recombinant Canine PD-1 Protein, hFc Tag (Cat. No. 70109-D02H) with an affinity constant of 44.1 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

The recombinant Canine CD274 consisting of 229 amino acids and has a calculated molecular mass of 26.18 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 48.1 kDa and 41.7 kDa protein in SDS-PAGE under reducing conditions.