CD22 Protein (C-His, Human)
Product Specifications
Abbreviation
CD22
Synonyms
SIGLEC-2, SIGLEC2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Anti-CD22 Antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human Siglec-2 / CD22 Protein (aa 176-687, His Tag) (Cat: 11958-H08H1), the EC50 is 8-25 ng/mL. 2.Loaded Anti-Human CD22 Antibody, Human IgG1 on AHC Biosensor, can bind Recombinant Human Siglec-2/CD22 Protein (aa 176-687), His Tag (Cat. No. 11958-H08H1) with an affinity constant of 5.92 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Monoclonal anti-Human Siglec-2 Antibody, human IgG1 captured on Protein A chip can bind Recombinant Human Siglec-2/CD22 Protein (aa 176-687), His Tag (Cat. No. 11958-H08H1) with an affinity constant of 2.147 nM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Trp176-Arg687
Molecular Weight
The recombinant human CD22 consists of 523 amino acids and predictes a molecular mass of 58.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the recombinant protein is approximately 90-100 kDa due to glycosylation.
Species
Human
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