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CD2 Protein (C-His, Human)

Product Specifications

Abbreviation

CD2

Synonyms

LFA-2, SRBC, T11

Host

HEK 293

Tag

C-His

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human CD2 Protein (ECD, His Tag) (Cat: 10982-H08H-UE) at 2 μg/mL (100 μL/well) can bind Recombinant Human LFA-3 / CD58 Protein (Fc Tag) (Cat: 12409-H02H), the EC50 is 75-225 ng/mL (Routinely tested) . 2.Loaded Recombinant Human LFA-3 / CD58 Protein (Fc Tag) (Cat. No. 12409-H02H) on proA Biosensor, can bind Recombinant Human CD2 Protein (ECD, His Tag) (Cat. No. 10982-H08H-UE) with an affinity constant of 2.32 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met 1-Asp 209

Molecular Weight

The recombinant human CD2 consists of 196 amino acids and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, it migrates as an approximately 36-40 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 31.3 kDa verified by SEC-MALS (Routinely tested) .

Species

Human

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