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CD19 Protein (C-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CD19

Synonyms

B4, CVID3

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

1.Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), the EC50 is 3.4-10 ng/mL (Routinely tested) . 2.Loaded Biotinylated Anti-CD19 (Research Grade Inebilizumab Biosimilar) on SA Biosensor, can bind Recombinant Human CD19 Protein, hFc Tag (Cat. No. 11880-H02W) with an affinity constant of 0.575 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human CD19 Protein (ECD, Fc Tag) (Cat: 11880-H02W) at 2 μg/mL (100 μL/well) can bind Anti-CD19 (Research Grade Inebilizumab Biosimilar), Biotinylated, the EC50 is 1-4 ng/mL (QC tested) .

Function

Met1-Lys291

Molecular Weight

The recombinant Human CD19 consists of 510 amino acids and predicts a molecular mass of 56.84 kDa. It migrates as an approximately 169.1 kDa band in SDS-PAGE under non-reducing conditions. The molecular weight of this protein is around 143.2 kDa verified by SEC-MALS (Routinely tested) .

Species

Human

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