CD14 Protein (C-His, Human)

Product Specifications

Abbreviation

CD14

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to enhance LPSstimulated IL8 secretion by THP1 human acute monocytic leukemia cells. The ED50 for this effect is typically 25-95 ng/mL.

Function

Met1-Met344

Molecular Weight

The secreted recombinant human CD14 consists of 336 amino acids and has a predicted molecular mass of 36.5 kDa. The apparent molecular mass of rhCD14 is approximately 45 kDa in SDS-PAGE under reducing conditions due to glycosylation.