CA12 Protein (C-His, Human)

Product Specifications

Abbreviation

CA12

Synonyms

CA-XII, CAXII, HsT18816, T18816

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.

Function

Met1-Gln291

Molecular Weight

The recombinant human CA12 consisting of 278 amino acids and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions.