C5 Protein (N-Flag & C-His, Human)

1.Loaded Eculizumab on ProA Biosensor, can bind Recombinant Human C5 Protein, His & FLAG Tag (Cat.No. 13416-H18H) with an affinity constant of 1.85nM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested) . 2.Immobilized Anti-C5 Antibody at 2 μg/mL (100 μL/well) can bind Recombinant Human C5 / Complement 5 Protein (Flag & His Tag) (Cat:13416-H18H), The EC50 is 25-75 ng/mL.

The recombinant human C5 consists of 1677 amino acids and predicts a molecular mass of 188.8 kDa. The C5 single chain can be processed into the disulfide-linked heterodimer of α and β chain with predicted molecular mass of 114 KDa and 74.8 kDa.