C1s Protein (C-His, Human)

Product Specifications

Abbreviation

C1s

Synonyms

C1S, EDSPD2

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB) . The specific activity is > 7,000 pmoles/min/μg, as measured under the described conditions.

Function

Met1-Asp688

Molecular Weight

The recombinant human C1s protease consists of 684 amino acids and has a predicted molecular mass of 76.3 kDa. As a result of glycosylation, rhC1s migrates as an approximately 82.06 kDa, 56.59 kDa and 33.36 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 88.5 kDa verified by SEC-MALS (Routinely tested) .