C1s Protein (C-His, Human)
Product Specifications
Abbreviation
C1s
Synonyms
C1S, EDSPD2
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90% as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-Lys-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB) . The specific activity is > 7,000 pmoles/min/μg, as measured under the described conditions.
Function
Met1-Asp688
Molecular Weight
The recombinant human C1s protease consists of 684 amino acids and has a predicted molecular mass of 76.3 kDa. As a result of glycosylation, rhC1s migrates as an approximately 82.06 kDa, 56.59 kDa and 33.36 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 88.5 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
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