AXL Protein (C-His, Human)

1.Label Recombinant Mouse GAS6 Protein (His Tag) ((Cat: 58026-M08H) with biotin. Immobilized Recombinant HumanAxl Kinase Protein (ECD, His Tag) (Cat: 10279-H08H) at 0.25 μg/mL (100 μL/well) can bind biotinylated Recombinant Mouse GAS6 Protein, the EC50 is 1.3-3.9 ng/mL (Routinely tested) . 2.Recombinant Human Axl Kinase Protein (ECD, His Tag) (Cat: 10279-H08H) immobilized on CM5 chip, can bind Recombinant Human GAS6 Protein (His Tag) (Cat: 13170-H07H3) with an affinity constant of 5.700 nM as determined in an SPR assay (Biacore 8K) (QC tested) .

The secreted recombinant human AXL consists of 428 amino acids after removal of the signal peptide and predicts a molecular mass of 46.5 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 60-70 kDa band due to glycosylation.