ACVR2B Protein (C-His, Human)

1.Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 0.1-1 μg/mL in the presence of 10 ng/mL recombinant Activin A (Routinely tested) . 2.Recombinant Human ACVR2B Protein, His Tag (Cat. No. 10229-H08H) immobilized on CM5 chip can bind Recombinant Mouse Activin A Protein, His Tag (Cat. No. 50659-M08H) with an affinity constant of 2.420 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) . 3.Recombinant Human ACVR2B / ActivinR-IIB Protein (His Tag) (Cat: 10229-H08H) immobilized on CM5 chip, can bind Recombinant Human/Mouse/Rat/Cynomolgus/Rhesus Activin A/INHBA Protein (Cat: 10429-HNAH) with an affinity constant of 0.239 nM as determined in an SPR assay (Biacore 8K) (QC tested) .

The recombinant human ACVR2B comprises 127 amino acids and predicts a molecular mass of 15 kDa. As a result of glycosylation, rh ACVR2B migrates as an approximately 33-38 kDa protein in SDS-PAGE under reducing conditions.