ACE2 Protein (C-human IgG1-Fc, Human)

1.Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 (2019-nCoV) Spike S1-His (Cat: 40591-V08B1) at 2 μg/mL (100 μL/well) can bind human ACE2 (hFc tag) (Cat: 10108-H02H), the EC50 of ACE2 is 5-35 ng/mL. 2.Captured ACE2 (10108-H02H on proA Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike S1 Protein (Cat.No.40591-V08H41) with an affinity constant of 11.78 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 3.Captured ACE2 (10108-H02H on anti-Human IgG Fc via CM5 Chip can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (Cat. No. 40592-V08H121) with an affinity constant of 5.667 nM as determined in a SPR assay (Biacore T200) (Routinely tested) . 4.Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein (Cat. No. 40592-V05H) on AMC Biosensor, can bind Recombinant Human ACE2 Protein , hFc Tag (Cat. No. 10108-H02H) with an affinity constant of 7.34 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human ACE2/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 961 amino acids and predicts a molecular mass of 110.3 kDa. As a result of glycosylation, the rhACE2/Fc monomer migrates as approximately 145-150 kDa band in SDS-PAGE under reducing conditions.