Spike Protein (C-His, MERS-CoV)
Product Specifications
Abbreviation
Spike
Host
HEK 293
Tag
C-His
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08H) at 2 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H) . The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 15-60 ng/ml.
Function
Met1-Glu725
Molecular Weight
The recombinant spike protein S1 Subunit MERS-CoV comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. As a result of glycosylation, it migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.
Species
MERS-CoV
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