Spike Protein (C-His, MERS-CoV)

Product Specifications

Abbreviation

Spike

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08H) at 2 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H) . The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 15-60 ng/ml.

Function

Met1-Glu725

Molecular Weight

The recombinant spike protein S1 Subunit MERS-CoV comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. As a result of glycosylation, it migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.