Spike Protein (C-His, MERS-CoV)

1. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08B1) at 10 μg/ml (100 μl/well) can bind biotinylated human DPP4 (Cat: 10688-HNCH) . The EC50 of of biotinylated DPP4 (Cat: 10688-HNCH) is 0.52-1.22 μg/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized Spike Protein S1 (aa 1-725) (Cat: 40069-V08B1) at 10 μg/ml (100 μl/well) can bind biotinylated Fc-DPP4 (Cat: 10688-H01H) . The EC50 of biotinylated Fc-DPP4 (Cat: 10688-H01H) is 0.02-0.06 μg/ml.

The recombinant spike protein S1 Subunit MERS-CoV comprises 719 amino acids and has a predicted molecular mass of 79.9 kDa. It migrates as an approximately 94 kDa band in SDS-PAGE under reducing conditions.