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RSV-F Protein (Native, RSV)

Product Specifications

Abbreviation

RSV-F

Host

CHO

Tag

Native

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS.

Bioactivity

1. Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (Site Ø) (Nirsevimab) at 2 μg/mL (100μL/well) can bind Recombinant RSVB Prefusion glycoprotein F/ RSV-preF Protein (Cat: 40999-VNAS), the EC50 is 4-12 ng/mL. 2. Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (site II) (Palivizumab) at 2 μg/mL (100μL/well) can bind Recombinant RSVB Prefusion glycoprotein F/ RSV-preF Protein (Cat: 40999-VNAS), the EC50 is 4-12 ng/mL. 3. Immobilized Anti-Fusion glycoprotein F0 Antibody, Human IgG1 (AM14) at 2 μg/mL (100μL/well) can bind Recombinant RSVB Prefusion glycoprotein F/ RSV-preF Protein (Cat: 40999-VNAS), the EC50 is 4-12 ng/mL. 4.Nirsevimab-derived anti-RSV-preF antibody, Human IgG1 captured on Protein A chip, can bind Human respiratory syncytial virus (RSVB) Prefusion glycoprotein F/ RSV-preF Protein (Cat. No. 40999-VNAS) with an affinity constant of 0.720 nM as determined in an SPR assay (Biacore 8K) (Routinely tested) .

Molecular Weight

It migrates as an approximately 58.2 kDa band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 160.8 kDa verified by SEC-MALS.

Species

RSV

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