NRP1 Protein (C-His, Human)

Product Specifications

Abbreviation

NRP1

Synonyms

BDCA4, CD304, NP1, NRP, VEGF165R

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1. Using the Octet RED System, the affinity constant (Kd) of NRP1-his bound to biotinylated human VEGF165 was 25nM (Routinely tested) . 2. Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat:11066-HNAH) at 2 μg/mL (100 μL/well) can bind Recombinant Human Neuropilin-1 / NRP1 / CD304 Protein (His Tag) (Cat:10011-H08H) with a linear range of 32-160 μg/mL (QC tested) . 3.Immobilized Recombinant Human Neuropilin-1 / NRP1 / CD304 Protein (His Tag) (Cat: 10011-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein, Biotinylated (Cat: 11066-HNAH-B), the EC50 is 174.2 ng/mL (Routinely tested) .

Function

Met1-Lys644

Molecular Weight

The secreted recombinant human NRP1 consists of 634 amino acids and has a calculated molecular mass of 71.3 kDa. As a result of glycosylation, rh NRP1 migrates as an approximately 88 kDa band in SDS-PAGE under reducing conditions.