NA Protein (C-His, H1N1)

Product Specifications

Abbreviation

Host

Baculovirus-insect Cells

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 90 % as determined by SDS-PAGE.

Bioactivity

1.Measured by its ability to cleave a fluorogenic substrate, 2'- (4-Methylumbelliferyl) -α-D-N-acetylneuraminic acid. The specific activity is > 8,000 pmoles/min/μg. 2.Immobilized Recombinant H1N1 (A/California/04/2009) NA (His Tag) (Cat: 11058-V08B) at 1 μg/mL (100 μL/well) can bind Anti-Influenza NA (Research Grade Clone 1G01), the EC50 is 2.5-8 ng/mL (QC tested) .

Function

His36-Lys469

Molecular Weight

The recombinant Influenza A virus (A/California/04/2009 (H1N1) ) NA consists of 480 amino acids and predicts a molecular mass of 53.6 kDa. As a result of glycosylation, it migrates as an approximately 58.7 kDa band in SDS-PAGE under reducing conditions.