MICA Protein (C-His, Human)

Product Specifications

Abbreviation

MICA

Synonyms

MIC-A, PERB11.1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 97 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized MICAh (Cat: 12302-H08H1) at 2 μg/mL (100 μL/well) can bind S4-Fc3L3-NKG2D (Cat: 10575-H01S), the EC50 of S4-Fc3L3-NKG2D (Cat: 10575-H01S) is 120-300 ng/mL.

Function

Met1-Gln308

Molecular Weight

The recombinant human MICA consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of human MICA is approximately 45-65 kDa due to glycosylation.