MET Protein (C-His, Cynomolgus, Rhesus)

Product Specifications

Abbreviation

MET

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 95% as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC.

Bioactivity

1.Immobilized Cynomolgus HGF (Cat:90286-CNAH) at 2 μg/ml (100 μl/well) can bind Cynomolgus c-MET His (Cat:90304-C08H), the EC50 of Cynomolgus c-MET His is 15-60 ng/mL (QC tested) . 2.Immobilized Recombinant Human HGF Protein (Cat: 10463-HNAS) at 2 μg/mL (100 μL/well) can bind Recombinant Cynomolgus / Rhesus c-MET / HGFR Protein (His Tag) (Cat: 90304-C08H), the EC50 is 183.5 ng/mL (Routinely tested) .

Function

Met1-Thr932

Molecular Weight

The recombinant cynomolgus / rhesus MET comprises 919 amino acids and has a calculated molecular mass of 103.1 KDa. The apparent molecular mass of it is approximately 128.2, 77.6 and 45.7 KDa in SDS-PAGE under reducing conditions.