IL4R Protein (C-His, Human)

1.Immobilized Recombinant Human IL4R / CD124 Protein (His Tag) (Cat: 10402-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL4 / Interleukin-4 Protein, Biotinylated (Cat: 11846-HNAE-B), the EC50 is 14-45 ng/mL (QC tested) . 2.Measured by its ability to inhibit IL-4-induced proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-25 ng/ml in the presence of 0.2 ng/mL of Recombinant human IL-4 (Routinely tested) . 3.Immobilized Recombinant Human IL4R / CD124 Protein (His Tag) (Cat: 10402-H08H) at 1 μg/mL (100 μL/well) can bind Anti-IL4R (Research Grade Stapokibart Biosimilar), the EC50 is 5.0 ng/mL (Routinely tested) .

The secreted recombinant human IL4R consists of 218 amino acids after cleavage of the signal peptide and predictes a molecular mass of 25.18 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43-48 kDa due to glycosylation.