IL13Ra2 Protein (C-His, Human)

1.Measured by its ability to inhibit IL13-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/mL (Routinely tested) . 2.Loaded Recombinant Human IL-13 Protein, hFc Tag (Cat. No. 10369-H01H) on ProA Biosensor, can bind Recombinant Human IL-13 R alpha 2/IL13RA2 Protein, His Tag (Cat. No. 10350-H08H) with an affinity constant of 1.99 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Anti- IL-13 R alpha 2/IL13RA2 antibody, human IgG1 on proA Biosensor, can bind Recombinant Human IL-13 R alpha 2/IL13RA2 Protein, His Tag (Cat. No. 10350-H08H) with an affinity constant of 4.640 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.Immobilized Anti- IL-13 R alpha 2/IL13RA2 antibody, human IgG1 at 2 μg/mL (100 μL/well) can bind Recombinant Human IL13RA2 / CD213A2 Protein (ECD, His Tag) (Cat: 10350-H08H), the EC50 is 1.0-4.0 ng/mL (QC tested) . 5.Immobilized Recombinant Human IL13 Protein (Fc Tag) (Cat: 10369-H01H) at 5 μg/mL (100 μL/well) can bind Recombinant Human IL13RA2 / CD213A2 Protein (ECD, His Tag) (Cat: 10350-H08H), the EC50 is 19.2 ng/mL (Routinely tested) . 6.Immobilized Recombinant Human IL13RA2 / CD213A2 Protein (ECD, His Tag) (Cat: 10350-H08H) at 1 μg/mL (100 μL/well) can bind anti- IL-13 R alpha 2/IL13RA2 antibody, human IgG1, the EC50 is 1.9 ng/mL (Routinely tested) .

The recombinant human IL13Rα2 consists of 325 amino acids and predicts a molecular mass of 38 KDa. As a result of glycosylation, it migrates as an approximately 49.7 kDa band in SDS-PAGE under reducing conditions.