IL12A & IL12B Protein (C-His, Human)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-His

Endotoxin

< 5 EU per mg of the protein.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL. The specific activity of recombinant human IL-12 is >10,000 Units/μg, which is calibrated against the human IL-12 WHO International Standard (NIBSC code: 95/544) .

Function

Met1-Ser219 & Met1-Ser328

Molecular Weight

The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.