IL12A & IL12B Protein (C-His, Human)

Product Specifications

Abbreviation

IL12A & IL12B

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Bioactivity

1.Immobilized Recombinant Human IL-12 R beta 2 / IL12RB2 Protein (ECD, His Tag) (Cat: 10145-H08H) at 5 μg/mL (100 μL/well) can bind Recombinant Human IL-12 (IL12A & IL12B Heterodimer) Protein, Biotinylated (Cat: CT011-H08H-B), the EC50 is 30-100 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human IL-12 (IL12A & IL12B Heterodimer) Protein, Biotinylated (Cat: CT011-H08H-B) at 1 μg/mL (100 μL/well) on streptavidin precoated (5 μg/mL, 100 μL/well) plate can bind Anti-IL-12 & IL-23 (P40) (Research Grade Ustekin*mab Biosimilar), the EC50 is 1.1-3.5 ng/mL (Routinely tested) .

Function

Met1-Ser219 & Met1-Ser328

Molecular Weight

The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.13 (23.99+36.14) kDa.

Species

Human