HMGB1 Protein (C-His, Human)

Product Specifications

Abbreviation

HMGB1

Synonyms

DKFZp686A04236, HMG-1, HMG1, HMG3, SBP-1

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

≥ 93 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

Immobilized Recombinant Human HMGB1 / HMG1 Protein (His Tag) (Cat: 10326-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human AGER / RAGE Protein (Fc Tag) (Cat: 11629-H02H), the EC50 is 30-100 ng/mL.

Function

Met1-Glu215

Molecular Weight

The recombinant human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.