FCGRT & B2M Protein (C-His, Human)
Product Specifications
Abbreviation
FCGRT & B2M
Host
HEK 293
Tag
C-His
Endotoxin
< 0.5 EU per mg protein.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .
Bioactivity
1.Loaded Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat. No. CT009-H08H-UE) on His1K Biosensor, can bind Anti-HER2 (Research Grade Trastuzumab Biosimilar) with an affinity constant of 6.138 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Recombinant Human FCGRT & B2M Heterodimer Protein (His Tag) (Cat: CT009-H08H-UE) captured on CM5 chip via Anti-His antibody, can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) with an affinity constant of 0.937 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Met1-Ser297 & Met1-Met119
Molecular Weight
The recombinant heterodimer of human FCGRT/B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.8+11.7) kDa. As a result of glycosylation, the bands migrate approximately 34-36 kDa and 12 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 48.4 kDa verified by SEC-MALS (Routinely tested) .
Species
Human
Curated Selection
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