FAP Protein (N-His, Human)

1.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC) . The specific activity is >1200 pmol/min/μg 2.Loaded anti-FAP antibody, human IgG1 on proA Biosensor, can bind Recombinant Human FAP Protein, His Tag (Cat. No. 10464-H07H) with an affinity constant of 0.396 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Immobilized Recombinant Human FAP / Seprase Protein (ECD, His Tag) (Cat: 10464-H07H) at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, human IgG1, the EC50 is 1.0-4.0 ng/mL (QC tested) .

The recombinant human FAP consists of 751 amino acids and predicts a molecular mass of 87.2 kDa. As a result of glycosylation, rh FAP migrates as approximately 90 kDa band in SDS-PAGE under reducing conditions.