CD70 Protein (N-human IgG1-Fc, Human)

Product Specifications

Abbreviation

CD70

Synonyms

CD27-L, CD27L, CD27LG, LPFS3, TNFSF7, TNLG8A

Host

HEK 293

Tag

N-human IgG1-Fc

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human CD27 His (Cat:10039-H08B1) at 2 μg/ml (100 μl/well) can bind Human CD70 hFc (Cat:10780-H01H), the EC50 of Human CD70 hFc is 15-60 ng/mL.

Function

Gln39-Pro193

Molecular Weight

The recombinant human CD70/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 413 amino acids and predicts a molecular mass of 45.5 kDa. As a result of different glycosylation, the rh CD70/Fc migrates as several bands with the approximate molecular mass of 55-60, 110-120 and 160-170 kDa corresponding to the monomeric, dimeric and trimeric form respectively in SDS-PAGE under reducing conditions.