CD3D & CD3E Protein (C-Flag & C-His, Human)
Product Specifications
Abbreviation
CD3D & CD3E
Host
HEK 293
Tag
C-Flag & C-His
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
Bioactivity
1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein, Biotinylated (Cat: CT038-H2508H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 antibody, Mouse IgG2a (Muromonab), the EC50 is 1-3 ng/mL. 2.Amine-Labeled Biotinylated Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat. No. CT038-H2508H-B) captured on CM5 chip via Anti-His antibody, can bind OKT3 derived Anti-CD3 antibody, Mouse IgG2a with an affinity constant of 0.241 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .
Function
Met1-Ala105 & Met1-Asp126
Molecular Weight
The recombinant human CD3D&CD3E consists of 271 (126+145) amino acids and predicts a molecular mass of 31.05 (14.4+16.65) KDa.
Species
Human
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