CD3D & CD3E Protein (C-Flag & C-His, Human)

1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein (Flag & His Tag) (Cat: CT038-H2508H) at 1 μg/mL (100 μL/well) can bind Anti-CD3 (OKT3) (Research Grade Muromonab Biosimilar), the EC50 is 5-15 ng/mL (QC tested) . 2.Mouse Anti-Human CD3 (SP34) captured on Protein A chip can bind Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat. No. CT038-H2508H) with an affinity constant of 22.120 nM as determined in an SPR assay (Biacore T200) (Routinely tested) . 3.Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat. No. CT038-H2508H) captured on CM5 chip via anti-His antibody, can bind OKT3 derived Anti-CD3 antibody, Mouse IgG2a with an affinity constant of 0.157 μM as determined in an SPR assay (Biacore T200) (Routinely tested) .

The recombinant heterodimer of human CD3D&CD3E comprises 271 (126+145) amino acids and has a calculated molecular mass of 31.05 (14.4+16.65) KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.