CD38 Protein (N-His, Human)

1.Flow cytometric analysis of anti-CD38 CAR expression. 5e5 of anti-CD38 CAR-293 cells were stained with 100 μL of 1:125 dilution of APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H85H-SA) and negative control protein. Non-transduced 293 cells were used as a control (left) . APC signal was used to evaluate the binding activity (QC tested) . 2.Binding activity of APC-conjugated CD38 protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and APC-conjugated human CD38 protein (Site-Specific APC Conjugation, ECD, His Tag, Cat. No. 10818-H85H-SA) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. APC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested) . 3.Flow cytometric analysis using APC conjugated CD38 protein. 5e5 of anti-CD38 CAR-293 cells were stained with APC-conjugated human CD38 protein (Site-Specific APC-Conjugated, ECD, His Tag, Cat. No. 10818-H85H-SA) . Non-transduced 293 cells were used as a control (Black Histogram) . The protein exhibited stable performance after multiple freeze-thaw cycles (5, 10 cycles) 4.Flow cytometric analysis using different lots of APC conjugated CD38 protein. 5e5 of anti-CD38 CAR-293 cells were stained with 2 lots of APC-conjugated human CD38 protein (Site-Specific APC-Conjugated, ECD, His Tag, Cat. No. 10818-H85H-SA) at the same concentration. Non-transduced 293 cells were used as a control (Black Histogram) . The results show high lot-to-lot consistency. 5.The site-specific APC conjugation specifically at the N-terminus (10818-H85H-SA) had good performance when binding to CAR that targeting epitopes distantal from the N-terminus (CAR02-293), and showed similar binding affinity for proximal C-terminal epitopes (CAR01-293) .