CD274 Protein (C-human IgG1-Fc, Human)

1.Immobilized Recombinant Human PD1 / PDCD1 Protein (His Tag) (Cat:10377-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat:10084-H02H), the EC50 is 220-660 ng/mL (Routinely tested) . 2.Immobilized Recombinant Human PD-L1 / B7-H1 / CD274 Protein (Fc Tag) (Cat: 10084-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human PD1 / PDCD1 Protein (Fc & AVI Tag), Biotinylated (Cat: 10377-H41H-B), the EC50 is 6-18 ng/mL (QC tested) . 3.Loaded Recombinant Human PD-1 Protein, His tag (Cat. No. 10377-H08H) on His1K Biosensor, can bind Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) with an affinity constant of 0.11 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 4.CHO cells were transduced with human PD1 and subjected to flow cytometric analysis using recombinant human PDL1 protein (Fc tag) (left, Cat. No. 10084-H02H, red) and a negative control protein (blue) . The cells were then stained with an FITC-conjugated anti-human IgG Fc antibody. Non-transduced CHO cells were used as a control (right) (Routinely tested) . 5.Loaded Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) on proA Biosensor, can bind Recombinant Human PD-1 Protein, His Tag (Cat. No. 10377-H08H) with an affinity constant of 8.110 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 6.Loaded Recombinant Human PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 10084-H02H) on proA Biosensor, can bind Recombinant Human B7-1/CD80 Protein, His Tag (Cat. No. 10698-H08H) with an affinity constant of 24.600 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 7. Immobilized Recombinant Human PD-L1/B7-H1 Protein (ECD, hFc Tag), HPLC-verified (Cat: 10084-H02H) at 5 μg/mL (100 μL/well) can bind Recombinant Human B7-1/CD80 Protein (hFc & AVI Tag), Biotinylated, HPLC-verified (Cat: 10698-H41H-B) the EC50 is 571 ng/mL (Routinely tested) .

The recombinant mature human B7-H1/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 459 amino acids and predicts a molecular mass of 52 kDa. As a result of glycosylation, the human B7-H1/Fc monomer migrates as an approximately 65-70 kDa protein in SDS-PAGE under reducing conditions.