CD274 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

CD274

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 0.5 EU per mg protein.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS (Routinely tested) .

Bioactivity

Loaded Recombinant Canine PD1 / PDCD1 / CD279 Protein (His Tag) (Cat. No. 70109-D08H) on His1K Biosensor, can bind Recombinant Canine PD-L1 / B7-H1 / CD274 Protein (ECD, Fc Tag) (Cat. No. 70110-D02H-UE) with an affinity constant of 12.2 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Arg236

Molecular Weight

The recombinant canine CD274 consists of 456 amino acids and predicts a molecular mass of 51.5 kDa. As a result of glycosylation, the band migrates approximately 70-80 kDa in the SDS-PAGE under reducing conditions. The molecular weight of this protein is around 130.9 kDa verified by SEC-MALS (Routinely tested) .