CD274 Protein (C-human IgG1-Fc, Canine)

Product Specifications

Abbreviation

CD274

Host

HEK 293

Tag

C-human IgG1-Fc

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.

Bioactivity

Loaded Recombinant Canine PD-1 Protein, His Tag (Cat. No. 70109-D08H) on His1K Biosensor, can bind Recombinant Canine PD-L1/B7-H1 Protein, hFc Tag (Cat. No. 70110-D02H) with an affinity constant of 38.1 nM as determined in BLI assay (Sartorius Octet RED384) (QC tested) .

Function

Met1-Arg236

Molecular Weight

The recombinant canine CD274 consists of 456 amino acids and predicts a molecular mass of 51.5 kDa. As a result of glycosylation, it migrates as an approximately 77.93 and 149.69 kDa band in SDS-PAGE under reducing conditions, corresponding to the monomer and homodimer.