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ANGPT2 Protein (C-His, Human)

Product Specifications

Abbreviation

ANGPT2

Synonyms

AGPT2, ANG2, LMPHM10

Host

HEK 293

Tag

C-His

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Purity

> 92 % as determined by SDS-PAGE

Bioactivity

1.Immobilized Recombinant Human ANG2 / Angiopoietin-2 Protein (His Tag) (Cat: 10691-H08H) at 2 μg/mL (100 μL/well) can bind Recombinant Human TIE-2 Protein (Fc Tag) (Cat: 10700-H02H), the EC50 is 200-600 ng/mL (QC tested) . 2.Loaded Recombinant Human TIE-2 Protein (hFc Tag) (Cat. No. 10700-H02H) on proA Biosensor, can bind Recombinant Human Angiopoietin-2/ANGPT2 Protein (His Tag) (Cat. No. 10691-H08H) with an affinity constant of 6.600 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 3.Loaded Recombinant Human TIE-2 Protein (hFc Tag) (Cat. No. 10700-H02H) on proA Biosensor, can bind Recombinant Human Angiopoietin-2/ANGPT2 Protein (His Tag) (Cat. No. 10691-H08H) with an affinity constant of 6.600 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

Function

Met1-Phe496

Molecular Weight

The recombinant human Ang2 consists of 489 amino acids and predicts a molecular mass of 56.4 kDa. As a result of glycosylation, the rhAng2 migrates as approximately 63.5 kDa band in SDS-PAGE under reducing conditions.

Species

Human

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