TSLP Protein (C-His, Human)

1.ImmobilizedRecombinant Human TSLP Protein (R127A, R130S, His Tag), Biotinylated (Cat: 16135-H08H-B) at 2 μg/mL (100 μl/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human CRLF2 Protein (ECD, Fc Tag) (Cat: 29749-H02H), the EC50 is 8-30 ng/mL (QC tested) . 2.Loaded Recombinant Human CRLF2/TSLPR Protein (hFc Tag) (Cat. No. 29749-H02H) on proA Biosensor, can bind Biotinylated Recombinant Human TSLP Protein (R127A, R130S, His Tag) (Cat. No. 16135-H08H-B) with an affinity constant of 42.000 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .

The recombinant human TSLP consists of 142 amino acids and predicts a molecular mass of 16.2 kDa. The molecular weight of this protein is around 25.7 kDa verified by SEC-MALS (Routinely tested) .